filmarray pneumonia panel mpcr  (BioFire Defense)

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Flow chart of the study population. ARF: acute respiratory failure; BFPP: BioFire® FilmArray® Pneumonia Panel; mPCR: multiplex polymerase chain reaction; ICU: intensive care unit; VAP: ventilation-acquired pneumonia

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Multiplex Assay, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: Incidence of bacteria detected by each of the 2 techniques BFPP mPCR: BioFire® FilmArray® Pneumonia Panel; CC: conventional culture; CNS: coagulase-negative Staphyloccocus ; PCR: polymerase chain reaction a Streptococcus except S.pneumoniae, S.agalactiae and S.pyogenes

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Bacteria, Polymerase Chain Reaction

Journal: Critical Care

Article Title: Diagnostic performance of Pneumonia multiplex PCR in critically ill immunocompromised patients

doi: 10.1186/s13054-025-05528-y

Figure Lengend Snippet: PCR and conventional culture results for BFPP panel bacteria. PCR: polymerase chain reaction; CC: conventional culture; TP: true positive; FP: false positive; FN: false negative; TN: true positive

Article Snippet: Though BioFire ® FilmArray ® Pneumonia Panel (BFPP) mPCR has been shown to have a good diagnostic performance in immunocompetent critically ill patients, little data exist in immunocompromised patients [ ].

Techniques: Bacteria, Polymerase Chain Reaction

Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Trial design. In the intervention arm, before the end of day 1, clinicians have to consider all the early microbiological results (mPCR FA-PPP, urine antigen assays, blood cultures and Gram stain examination of respiratory tract sample) and procalcitonin before the end of day 1, and subsequently to apply an algorithm of early antibiotics discontinuation or de-escalation (red square). This algorithm is described in . In both arms, at day 3 and day after day until day 7, clinicians are encouraged to consider antibiotic discontinuation, based on procalcitonin values and kinetics. CAP, community-acquired pneumonia; FA-PPP, FilmArray Pneumonia Panel Plus; ICU, intensive care unit; mPCR, multiplex PCR.

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Staining, Multiplex Assay

Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Algorithm of early antibiotics de-escalation or discontinuation. The algorithm is applied in the intervention arm only (see red square, ). mPCR, multiplex PCR; ICU, intensive care unit; PCT, procalcitonin.

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Multiplex Assay

Journal: BMJ Open

Article Title: Combined use of a broad-panel respiratory multiplex PCR and procalcitonin to reduce duration of antibiotics exposure in patients with severe community-acquired pneumonia (MULTI-CAP): a multicentre, parallel-group, open-label, individual randomised trial conducted in French intensive care units

doi: 10.1136/bmjopen-2020-048187

Figure Lengend Snippet: Primary and secondary outcomes

Article Snippet: In the experimental strategy arm, the microbiological diagnosis combines a broad-panel respiratory mPCR (FilmArray Pneumonia Panel Plus (FA-PPP), BioFire Biomérieux) with conventional microbiological investigations.

Techniques: Bacteria, Biomarker Discovery